A fast, miniaturised in-vitro assay developed for quantification of lipase enzyme activity
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چکیده
منابع مشابه
application and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples
ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
15 صفحه اولactivity assay of glutathione s-transferase (gsts) enzyme as a diagnostic biomarker for liver hydatid cyst in vitro.
the aim of this study was to detect the glutathione s-transferase(gst) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. in order to compare with liver tissue, the level of the gsts enzyme activity of parasite was also determined.parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with pbs buffer. collected parasi...
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Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for epidemiologic studies. The present study was performed with a commercial human en...
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A noncompetitive enzyme-linked immunosorbent assay to measure rat hepatic triglyceride lipase (H-TGL) was developed. Antibodies to rat H-TGL were purified from goat antisera by immunoadsorption on an H-TGL-Sepharose 4B column. Routinely, Immulon 2 Removawell strips were coated with the purified antibody overnight at 4 degrees C. After blocking the wells with bovine serum albumin (BSA) for 2 hr ...
متن کاملDirect fluorescence-based lipase activity assay.
Lipases and esterases (glycerol ester hydrolases, E.C. 3.1.1.-) are enzymes acting on the carboxyl ester bonds present in acylglycerols. They have been recognized as useful biocatalysts because of their wide-ranging versatility in industrial applications (2,5). However, the physical properties of lipids have caused a great difficulty in studying lipolytic enzymes (4). Traditionally, lipases hav...
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ژورنال
عنوان ژورنال: Journal of Enzyme Inhibition and Medicinal Chemistry
سال: 2019
ISSN: 1475-6366,1475-6374
DOI: 10.1080/14756366.2019.1651312